Expression of Cdk5 and its activators in NT2 cells during neuronal differentiation

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Expression of Cdk5 and its activators in NT2 cells during neuronal differentiation.

We have recently developed a rapid protocol involving NT2 cell aggregation and treatment with retinoic acid (RA) to produce terminally differentiated CNS neurons. As a first step to explore the functional roles of cell-cycle regulatory proteins in the process of neuronal differentiation, the expression profiles of cyclin-dependent kinases (Cdks) and their regulators were examined in NT2 cells f...

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Expression and activity of cyclin-dependent kinases and glycogen synthase kinase-3 during NT2 neuronal differentiation.

In the presence of retinoic acid undifferentiated NT2 cells turn into terminally differentiated hNT (or NT2N) neurons within 5 weeks. We have used this in vitro cellular model to investigate the changes in expression and activity of cyclin-dependent kinases (CDKs) and glycogen synthase kinase-3 (GSK-3) during this neuronal differentiation process. We here show that CDK1/2 protein level and kina...

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ژورنال

عنوان ژورنال: Journal of Neurochemistry

سال: 2002

ISSN: 0022-3042

DOI: 10.1046/j.1471-4159.2002.00856.x